p-s6 (ser235/236; clone: d57.2.2e) antibody (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
p-s6 (ser235/236; clone: d57.2.2e) antibody
P S6 (Ser235/236; Clone: D57.2.2e) Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p-s6+(clone+d57%2E2%2E2e)+antibody/rabbit+anti+ps6/pm36800413-330-21-25
Average 90 stars, based on 1 article reviews
P S6 (Ser235/236; Clone: D57.2.2e) Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p-s6+(clone+d57%2E2%2E2e)+antibody/rabbit+anti+ps6/pm36800413-330-21-25
Average 90 stars, based on 1 article reviews
p-s6 (ser235/236; clone: d57.2.2e) antibody - by Bioz Stars,
2026-09
90/100 stars
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Staining:Article Title: CD38 in SLE CD4 T cells promotes Ca 2+ flux and suppresses interleukin-2 production by enhancing the expression of GM2 on the surface membrane Article Snippet: The phenotype of T cell subpopulations was measured by flow cytometry, using fluorochrome-conjugated antibodies against CD3 (Clone: UCHT1, #300439, Biolegend), CD4 (Clone: RPA-T4, #300528, Biolegend), CD8 (Clone: HIT8a, #300907, Biolegend), CD38 (Clone: HIT2, #303516, Biolegend), CTB (C1655, Sigma Aldrich), CD45RA (Clone:HI100, #304118, Biolegend), CCR7 (Clone:G043H7, #353212, Biolegend), IL-2 (Clone: MQ1-17H12, #500326, Biolegend), IFNγ (Clone: 4S.B3, 502521, Biolegend), TNF (Clone: MAb11, #502913, Biolegend), IL-4 (Clone: MP4-25D2, #500811, Biolegend), IL-6 (Clone: MQ2-13A5, #501107, Biolegend), IL-10 (Clone:JES3-9D7, #50-7108-42, eBioscience), IL-13 (Clone: JES10-5A2, #501903, Biolegend), IL-17 (Clone; eBio64DEC17, #17-7179-42, eBioscience), XBP1s (Clone: 143 F, #647504, Biolegend) with proper isotypes as controls (PE/Cy7 Isotype control, Clone:MOPC-21, #400126, Biolegend). .. For intracellular staining, cells were fixed and permeabilized using a Cytofix/Cytoperm kit (#554714, BD Biosciences) to stain cytoplasmic proteins or using a True Nuclear Transcription Factor buffer set (#424401, Biolegend) to stain B4GALNT1 (Clone:M02, #AT1255a, Abcepta, 1:50), HEXA (Clone: #MAB6237-SP, R&D, 1:50), ST3GAL5 (Clone:aa389-418, #LS-C161530, LSBio,1:50), GM3 (Clone: GMR6, #A2582, TCI, 1:50), GM2A (Clone:06, # 13246-MM06, Sino Biological, 1:50), GM2 (Clone:06, #A2575, TCI, 1:50), GD1a (Clone: GD1a-1, #MAB5606Z, TCI, 1:50), pCD3ζ (Clone: 6B10.2, # 644103, Biolegend, 1:50), pZAP70 (Clone: 1503310 #683703, Biolegend, 1:50), pPLCγ1 (Clone: 3H1C10, # NBP2-52533, Novus, 1:50), pPLCγ1-Tyr783 (FITC, PLCG1Y783-C4, #MA5-28029, Invitrogen), PLCγ1 (#LS-C669421, LSBio), pLCK (Clone: SRRCHA, #46-9076-41, eBioscience), pZAP70/Syk (Clone:, #12-9006-41, eBioscience), pSHP1 (Clone:P1C1-A5, #656603, Biolegend), pSHP2 (Clone: L99-921, #L99-921, BD), pJNK (Clone:N9-66, #562480, BD), pMAPK (Clone: 4NIT4KK, #12-9078-42, eBioscience), pERK (Clone:4B11B6, #675503, Biolegend), pAKT (Clone:D25E6, #13038S, CST), Article Title: The MARK2 kinase acts as a gatekeeper of CD28-dependent co-stimulation in T cells Article Snippet: Cells were washed in FACS buffer and permeabilized in BD PhosflowTM Perm Buffer III (BD Biosciences, catalog no. 558050) for 30 min at 4°C. .. After washing, intracellular staining was performed for 45min at 4°C with Article Title: CD38 in SLE CD4 T cells promotes Ca 2+ flux and suppresses interleukin-2 production by enhancing the expression of GM2 on the surface membrane. Article Snippet: The phenotype of T cell subpopulations was measured by flow cytometry, using fluorochrome-conjugated antibodies against CD3 (Clone: UCHT1, #300439, Biolegend), CD4 (Clone: RPAT4, #300528, Biolegend), CD8 (Clone: HIT8a, #300907, Biolegend), CD38 (Clone: HIT2, #303516, Biolegend), CTB (C1655, Sigma Aldrich), CD45RA (Clone:HI100, #304118, Biolegend), CCR7 (Clone:G043H7, #353212, Biolegend), IL-2 (Clone: MQ1-17H12, #500326, Biolegend), IFNγ (Clone: 4S.B3, 502521, Biolegend), TNF (Clone: MAb11, #502913, Biolegend), IL-4 (Clone: MP4-25D2, #500811, Biolegend), IL-6 (Clone: MQ2-13A5, #501107, Biolegend), IL-10 (Clone:JES3-9D7, #50-7108-42, eBioscience), IL-13 (Clone: JES10-5A2, #501903, Biolegend), IL-17 (Clone; eBio64DEC17, #17-7179-42, eBioscience), XBP1s (Clone: 143 F, #647504, Biolegend) with proper isotypes as controls (PE/Cy7 Isotype control, Clone:MOPC-21, #400126, Biolegend). .. For intracellular staining, cells were fixed and permeabilized using a Cytofix/Cytoperm kit (#554714, BD Biosciences) to stain cytoplasmic proteins or using a True Nuclear Transcription Factor buffer set (#424401, Biolegend) to stain B4GALNT1 (Clone:M02, #AT1255a, Abcepta, 1:50), HEXA (Clone: #MAB6237-SP, R&D, 1:50), ST3GAL5 (Clone:aa389-418, #LS-C161530, LSBio,1:50), GM3 (Clone: GMR6, #A2582, TCI, 1:50), GM2A (Clone:06, # 13246-MM06, Sino Biological, 1:50), GM2 (Clone:06, #A2575, TCI, 1:50), GD1a (Clone: GD1a-1, #MAB5606Z, TCI, 1:50), pCD3ζ (Clone: 6B10.2, # 644103, Biolegend, 1:50), pZAP70 (Clone: 1503310 #683703, Biolegend, 1:50), pPLCγ1 (Clone: 3H1C10, # NBP2-52533, Novus, 1:50), pPLCγ1-Tyr783 (FITC, PLCG1Y783-C4, #MA5-28029, Invitrogen), PLCγ1 (#LS-C669421, LSBio), pLCK (Clone: SRRCHA, #46-9076-41, eBioscience), pZAP70/Syk (Clone:, #12-9006- 41, eBioscience), pSHP1 (Clone:P1C1-A5, #656603, Biolegend), pSHP2 (Clone: L99-921, #L99-921, BD), pJNK (Clone:N9-66, #562480, BD), pMAPK (Clone: 4NIT4KK, #12-9078-42, eBioscience), pERK (Clone:4B11B6, #675503, Biolegend), pAKT (Clone:D25E6, #13038S, CST), Article Title: Glutamine availability regulates cDC subsets in tissue Article Snippet: .. Biolegend: CD45R/B220 – APC/Cy7 (Clone RA3-6B2, # 103224), BV510 (Clone RA3-6B2, # 103247); CD8α – AF647 (Clone 53-6.7, # 100724), PE/Dazzle 594 (Clone 53-6.7, # 100762); CD11c – APC/Cy7 (Clone N418, # 11), PE-Dazzle-594 (Clone N418, # 117348); CD24 – APC (Clone M1/69, # 101814); CD45 – BV510(Clone IM7, # 103043), BV605 (Clone 30-F11, # 103139); CD64 – PE (Clone X54-5/7.1, #139304); CD103 – AF647 (Clone 2E7, #121410), PE/Dazzle 594 (Clone 2E7, #121430); CD172α/SIRPα – PerCP/Cy5.5 (Clone P84, # 144009), BV510 (Clone P84, #144302), FITC (Clone P84, # 144006); I-A/I-E 1:400 – BV421 (Clone M5/114.15.2, # 107631); Ly6c – BV605 (Clone HK1.4, #128036), PE (Clone HK1.4, #128007); Ly6g 1:400 – PerCP/Cy5.5 (Clone 1A8, # 127616); F4/80 – BV605 (Clone BM8, # 123133); XCR1 – BV510 (Clone ZET, # 148218) Invitrogen: CD11b 1:400 – PE/Cy7 (Clone M1/70, # 25-0112-82), BUV805 (Clone M1/70, # 368-0112-82) BD: CD45 - BUV395 (Clone 30-F11, # 564279); CD135 1:100 for 60 minutes at 4 C – PE-CF594 (Clone 562537, # A2F10.1); CD117 1:100 for 60 minutes at 4 C – BUV395 (Clone 2B8, # 564011); Ki67 stained intracellularly – PE/Cy7 (Clone B56, # 561283); Active Caspase 3 stained intracellularly – FITC (Clone C92-605, # 560901) Cell signaling: FACS:Article Title: The MARK2 kinase acts as a gatekeeper of CD28-dependent co-stimulation in T cells Article Snippet: Cells were washed in FACS buffer and permeabilized in BD PhosflowTM Perm Buffer III (BD Biosciences, catalog no. 558050) for 30 min at 4°C. .. After washing, intracellular staining was performed for 45min at 4°C with other:Article Title: B cell adapter for PI 3-kinase (BCAP) coordinates antigen internalization and trafficking through the B cell receptor Article Snippet: Antibodies for microscopy included the following: anti–NF-κB p65 (D14E12, catalog no. 8242), pERK (20G11, catalog no. 4376S), Rab5 (C8B1, catalog no. 3547S), and pS6k (AF647 conjugated, clone D57.2.2E, catalog no. 4851) from Cell Signaling Technology; anti-pTyr AF488 conjugated (catalog no. 309306) from BioLegend; and LAMP1 antibody (catalog no. BDB5537) purchased from Thermo Fisher Scientific. |